: 4732

MethoCult™ H4034 Optimum

: 1478839273

MethoCult™ H4034 Optimum

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    MethoCult™ H4034 Optimum

    Methylcellulose-based medium with recombinant cytokines for human cells

    Overview

    MethoCult™ H4034 Optimum (MethoCult™ GF H4034) is a methylcellulose-based medium for the growth and enumeration of hematopoietic progenitor cells in colony-forming unit (CFU) assays of human bone marrow, mobilized peripheral blood, peripheral blood, and cord blood samples. MethoCult™ H4034 Optimum has been formulated to support optimal growth of erythroid progenitor cells (BFU-E and CFU-E), granulocyte-macrophage progenitor cells (CFU-GM, CFU-G and CFU-M), and multipotential granulocyte, erythroid, macrophage, megakaryocyte progenitor cells (CFU-GEMM). The inclusion of G-CSF in Optimum formulations improves discrimination of myeloid colony subtypes. This formulation is compatible with STEMvision™ software for automated colony counting.
    Contains:
    • Methylcellulose in Iscove's MDM 
    • Fetal bovine serum 
    • Bovine serum albumin 
    • 2-Mercaptoethanol 
    • Recombinant human stem cell factor (SCF) 
    • Recombinant human interleukin 3 (IL-3) 
    • Recombinant human erythropoietin (EPO) 
    • Recombinant human granulocyte colony-stimulating factor (G-CSF) 
    • Recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF) 
    • Supplements
    Subtype:
    Semi-Solid Media; Specialized Media
    Cell Type:
    Hematopoietic Stem and Progenitor Cells
    Species:
    Human; Non-Human Primate
    Application:
    Cell Culture; Colony Assay; Functional Assay
    Brand:
    MethoCult
    Area of Interest:
    Drug Discovery and Toxicity Testing; Stem Cell Biology